The Secretariat accepts students on weekdays from 12:00 - 13:30



Study Guide 2020-21

Spyroulias George PDF Print
Tel. :(+30) 2610 962350 Office
        (+30) 2610 962351 Lab / Postgraduate Students 
        (+30) 2610 962352 NMR

Fax : (+302610) 997693
E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Scopus Author ID:  6602122371

Google Scholar ID: Go to Link

ORCID ID: 0000-0003-1799-3312    

Recent CV

Current Focus of Research

• Design/Simulation of bioactive molecules of pharmaceutical interest and Conformational Analysis of biomolecules through Nuclear Magnetic Resonance Spectroscopy (ΝΜR).
• Structural Bioinformatics applied in homology modeling of unknown structures of biomolecules, protein-protein/peptide/substrate interaction through molecular dynamics simulation, docking simulation protocols.
• In silico design and development of combinatorial libraries of compounds and virtual screening process towards the quest of lead-molecules with biological activity towards protein/enzyme targets, etc.


  1. Tsika, S. Melekis, S.-A. Tsatsouli, N. Papageorgiou, M.J. Maté, B. Canard, B. Coutard, D. Bentrop, G.A. Spyroulias. Deciphering the nucleotide and RNA binding selectivity of the Mayaro virus macro domain. (2019) Journal of Molecular Biology 431, 2282-2296, (2019).
  2. G.I. Makrynitsa, D. Ntonti, K.D. Marousis, M. Birkou, M.-T. Matsoukas, S. Asami, D. Bentrop, N. Papageorgiou, B. Canard, B. Coutard, G.A. Spyroulias. Conformational plasticity of the VEEV macro domain is important for binding of ADP-ribose. Journal of Structural Biology, 206, 119-127, (2019). 
  3. M.S. Twigg, L. Tripathi, A. Zompra, K. Salek, V.U. Irorere, T. Gutierrez, G.A. Spyroulias, R. Marchant, I.M. Banat. Identification and Characterisation of Short Chain Rhamnolipid Production in a Previously Uninvestigated, Non-Pathogenic Marine Pseudomonad. Applied Microbiology and Biotechnology 102, 8537–8549, (2018). 
  4. K.D. Marousis, A.C. Tsika, M. Mbirkou, M.-T. Matsoukas, G.A. Spyroulias. Lead Identification through the synergistic action of Biomolecular NMR and in silico methodologies. Methods in Molecular Biology 1824, 299-316, (2018). 
  5. D.S.M. Chatzileontiadou, A.C. Tsika, Z. Diamantopoulou, J. Delbé, J. Badet, J. Courty, V. Parmenopoulou, D. Komiotis, J.M. Hayes, G.A. Spyroulias, D.D. Leonidas. Triazole pyrimidine nucleosides are potent inhibitors of human Angiogenin in vivo. Evidence for novel action at the cell binding site revealed by heteronuclear NMR spectroscopy and modelling studies. ChemMedChem 13, 259-269, (2018). 
  6. E. Zvintzou, M. Lhomme, S.A. Chasapi, S. Filou, V. Theodoropoulos, E. Xapapadaki, A. Kontush, G.A. Spyroulias, K.E. Kypreos. Pleiotropic Effects of Apolipoprotein C3 on HDL Functionality and Adipose Tissue Metabolic Activity. Journal of Lipid Research 58, 1869-1883, (2017). 
  7. M. Birkou, C.T. Chasapis, K.D. Marousis, A.K. Loutsidou, D. Bentrop, M. Leli, T. Herrmann, Carthy JM, V. Episkopou, G.A. Spyroulias. A residue-specific insight into the Arkadia E3 ubiquitin ligase activity and conformational plasticity. Journal of Molecular Biology 429, 2373-2386 (2017). 
  8. M. Suarez-Diez, J. Adam, J. Adamski, S.A. Chasapi, C. Luchinat, A. Peters, C. Prehn, C. Santucci, A. Spyridonidis, G.A. Spyroulias, L. Tenori, R. Wang-Sattler, E. Saccenti. Plasma and serum metabolite association networks: comparability within and between studies using NMR and MS profiling. Journal of Proteome Research 16, 2547-2559, (2017). 
  9. D. Liourdi, P. Kallidonis, I. Kyriazis, A. Tsamandas, D. Karnabatidis, P. Kitrou, G.A. Spyroulias, O.N. Kostopoulou, K. Marousis, D.L. Kalpaxis, D.S. Goumenos, E. Liatsikos. Evaluation of the distribution of Paclitaxel by Immunohistochemistry and Nuclear Magnetic Resonance Spectroscopy after the application of Drug-eluting balloon in porcine ureter. Journal of Endourology 29, 580-589 (2015). 
  10. T. Katsila, G.A. Spyroulias, G.P. Patrinos, M.-T. Matsoukas. Computational approaches in target identification and drug discovery. Computational and Structural Biotechnology Journal 14, 177-84, (2016). 
  11. I. Sharina, M. Sobolevsky, A. Papakyriakou, N. Rukoyatkina, G.A. Spyroulias, S. Gambaryan, E. Martin. The fibrate gemfibrozil is an NO- and heme-independent activator of soluble guanylyl cyclase: in vitro studies. British Journal of Pharmacology 172, 2316-2329 (2015). 
  12. M. von Wantoch Rekowski, V. Kumar, Z. Zhou,; M. Bantzi, A. Marazioti, J. Moschner, G.A. Spyroulias, F. van den Akker, A. Giannis, A. Papapetropoulos. Insights into soluble guanylyl cyclase activation derived from improved heme-mimetics. Journal of Medicinal Chemistry 56, 8948–8952 (2013). 
  13. Abriata LA, Banci L, Bertini I, Ciofi-Baffoni S, Gkazonis P, Spyroulias GA, Vila AJ, Wang S. Mechanism of Cu(A) assembly. Nat Chem Biol. 4, 599-601 (2008). 
  14. A. Papakyriakou, G.A. Spyroulias, E. Sturrock, E. Manessi-Zoupa, P. Cordopatis. Simulated Interactions between Angiotensin-Converting Enzyme and Substrate Gonadotropin-Releasing Hormone: Novel Insights into Domain-Selectivity. Biochemistry 46, 8753-8765 (2007). 
  15. L. Banci, I. Bertini, S. Ciofi-Baffoni, N.G. Kandias, G.A. Spyroulias, N.J. Robinson, M. Vanarotti, X.-C. Su. The delivery of copper for thylakoid import observed by NMR. Proc. Natl Acad Sci. USA 103, 8320-8325 (2006). 

Tuesday 11:00-14:00 Thursday 11:00-14:00 Wednesday 11:00-14:00  

For more information visit the group web site:

Design / Simulation, NMR Spectroscopy & Conformational Analysis of Bioactive compounds