Secretariat Time Programme
Daily
12:00 - 13:30

sfe

Laboratory of Instrumental Pharmaceutical Analysis

Laboratory of Instrumental Pharmaceutical Analysis People Academic

Staff

Graduate Students and PhD Candidates

Alumni

  • Dr Dionysios Douroumis, Senior Lecturer in Pharmaceutical Sciences, University of Greenwich
  • Dr Fotini Bazoti, Researcher, GAIA Center, The Goulandris Natural History Museum
  • Dr John Karampas, QC/QA Director, TSAKIRIS Food and Snacks
  • Dr Nikolaos Vayenas, Quality Control of Products, Ministry of Development
  • Dr George Carountzos, QC Director, EBO-PYRKAL
  • Kostas Kalonakis, Medical/Pharmaceutical Representative, Uni-Pharma
  • Dimitra Filippatou, Pharmacy
  • Nikos Charalabopoulos,Pharmacy
  • Catherine Ntzelve, Pharmacy
  • Maria Makriyorgi, Pharmacy
  •  Mohamed Ahmed El Mubarak, Translator, Agios Andreas Hospital

Research Interests / Activities

  • Drug metabolism and pharmakokinetic studies, bioavailability studies of drugs and natural bioactive substances.
  • Analysis of complex biological systems and structural characterization of natural and biotechnology-derived biomolecules (peptides, proteins).
  • Detection, structural characterization, identification and quantitative determination of bioactive substances isolated from native plants.
  • Study of the interaction between natural products and endogenous molecules responsible for certain diseases (i.e., Alzheimer disease).
  • Detection of non-covalent complexes of biomolecules and bioactive molecules, as well as mapping of the binding sites.
  • Development of novel non-destructive analytical methodologies for:
    • The qualitative and quantitative determination of pharmaceutically active substances:
      • In pharmaceutical formulations (liquid or tablets),
      • In biological fluids,
      • For the study of drug release from micro- and nano-particles
    • The characterization of polymorphic phases and generic drugs
    • The study / monitoring of the chemical alterations caused during diseases of bone and cartilage (osteoporosis, osteoarthritis)

Current Academic Collaborations

  1. Universities
    • University of Patras (Departments of Medicine, Chemical Engineering, Mechanical Engineering and Aeronautics, Chemistry)
    • National and Kapodistrian University of Athens (Medical School & Department of Pharmacy)
    • University of Crete (Medical School)
    • Agricultural University of Athens
    • Aristotle University of Thessaloniki (School of Pharmacy)
    • National Technical University of Athens (School of Chemical Engineering)
    • University of Michigan (Department of Chemistry)
    • University of Frankfurt (Pharmacy Department)
    • Uppsala University (Department of Chemistry)
    • Chalmers University of Technology
    • TU WIEN (Institute of Chemical Technologies and Analytics)
  2. Β. Research Institutes
    • ICEHT/FORTH
    • GAIA Center, Goulandris Natural History Museum
    • INRA Theix (Groupe Ostéoporose Unité des Maladies Métaboliques et Micronutriments)
    • Scripps Research Institute (Department of Chemistry)

Advanced Research – Product Development

Our lab collaborates with pharmaceutical companies GENEPHARM, Galenica, Specifar, PharOS, Pharmadata, Verisfield UK Ltd, Pharmex for issues concerning:

  • Development of new formulations
  • Testing of APIs polymorphism in tablets and creams
  • Physicochemical characteristics testing
  • Quantitative determination of active substances in biological fluids

Financially Supported Research Programs and Networks

  • «K. Karathodori» (M. Orkoula), University of Patras
  • «Thalis» (Ch. Kontoyannis), General Secretariat for Research and Technology
  • «Heracleitus» (Ch. Kontoyannis), General Secretariat for Research and Technology
  • «Intra-University Research Network» with Departments of Medicine, Chemistry, Mechanical Engineering and Aeronautics, entitled: Network for the study of retrogressive and metabolic disoorders of bone and cartilage – OSTEONET (Ch. Kontoyannis, M. Orkoula)
  • «Thalis» (A. Tsarbopoulos), General Secretariat for Research and Technology

Publications

  • N.S. Koulakiotis, E. Pittenauer, M. Halabalaki, A. Tsarbopoulos and G. Allmaier, “Comparison of different tandem mass spectro-metric techniques (ESI-IT, ESI- and IP-MALDI-QRTOF and VMALDI-TOF/RTOF) for the analysis of crocins and picrocrocin from the stigmas of crocus sativus l.”, Rapid Commun. Mass Spectrom. 2012, 26, 670-678.
  • F.N. Bazoti, E. Gikas, A. Skoutelis and A. Tsarbopoulos, “Development and Validation of an Ultra Performance Liquid Chromatography – Tandem Mass Spectrometry Method for the Quantification of Daptomycin in Human Plasma”, J. Pharm. Biomed. Analysis 2011, 56, 78-85.
  • F.N. Bazoti, J. Bergquist, K. Markides and A. Tsarbopoulos, “Localization of the Binding Site in the Non-Covalent Interaction between Amyloid-β Peptide (1-40) and Oleuropein Using Electrospray Ionization FTICR Mass Spectrometry”, J. Am. Soc. Mass Spectrom. 2008, 19, 1078-1085.
  • S.S. Petropoulou, E. Gikas, A. Tsarbopoulos and P. Siskos, “Gas Chromatographic-Tandem Mass Spectrometric Method for the Quantitation of Carbofuran, Carbaryl and their Main Metabolites in Applicators’ Urine”, J. Chromatogr. A 2006, 1108, 99-110.
  • F.N. Bazoti, J. Bergquist, K. Markides and A. Tsarbopoulos, “Detection of the Non-Covalent Complex between Amyloid-β Peptide (1-40) and Oleuropein using Electrospray Ionization Mass Spectrometry”, J. Am. Soc. Mass Spectrom. 2006, 17, 568-575.
  • F.N. Bazoti, A. Tsarbopoulos, K. Markides and J. Bergquist, “Study of the Non-Covalent Interaction between Amyloid-ί Peptide and Melatonin using Electrospray Ionization Mass Spectrometry”, J. Mass Spectrom. 2005, 40, 182-192.
  • F.N. Bazoti, E. Gikas, C. Puel, V. Coxam and A. Tsarbopoulos, “Development of a Sensitive and Specific SPE-GC-MS/MS Method for the Determination of Elenolic Acid, Hydroxytyrosol and Tyrosol in Rat Urine”, J. Agr. Food Chem. 2005, 53, 6213-6221.
  • E. Gikas, P. Kormali, D. Tsipi and A. Tsarbopoulos “Development of a Rapid and Sensitive SPE-LC-ESI MS/MS Method for the Determination of Chloramphenicol in Seafood”, J. Agr. Food Chem. 2004, 52, 1025-1030.
  • A. Tsarbopoulos, U. Bahr, B.N. Pramanik and M. Karas, “Structure Analysis of Glycoproteins by Electrospray Ionization Mass Spectrometry” in “Applied Electrospray Mass Spectrometry”, Marcel Dekker, New York, 2002, 283-306.
  • A.K. Ganguly, B.N. Pramanik, G. Chen and A. Tsarbopoulos, “Detection of Non-covalent Complexes by Electrospray Ionization Mass Spectrometry” in “Applied Electrospray Mass Spectrometry”, Marcel Dekker, New York, 2002, 361-387.
  • D. Skorda and C. Kontoyannis, "Identification and Quantitative Determination of Atorvastatin Calcium Polymorph in Tablets Using FT-Raman Spectroscopy” Talanta 74 (2008) 1066
  • I.A. Karampas, M.G. Orkoula and C.G. Kontoyannis, «Effect of Hydrazine Based Deproteination Protocol on Bone Mineral Crystal Structure» J. Mater. Sci.: Mater. Med., 23 (2012) 1139-1148.
  • M. Orkoula, M. Friedman, D. Kohn and M. Morris, «Effect pf Exercise and Nutrition on Composition of Murine Tibia» Bone, 48 (2011) S179 Suppl.2.
  • C.T. Chasapis, A.K. Loutsidou, M.G. Orkoula and G.A. Spyroulias, «Zinc Binding Properties of Engineered RING Finger Domains of Arkadia E3 Ubiquitin Ligase» Bioinorganic Chemistry and Applications, 2010 (2010) Article ID 323152.
  • M.G. Orkoula, C. Kontoyannis, «Non-Destructive Quantitative Analysis of Risperidone in Film-Coated Tablets», J. Pharm. Biomed. Anal., 47 (2008) 251-257.
  • C. K. Markopoulou, J.E. Koundourellis, M.G. Orkoula and C. G. Kontoyannis, «Quantitative Non-Destructive Methods for the Determination of Ticlopidine in Tablets Using Reflectance Near-Infrared and Fourier-Transform Raman Spectroscopy», Applied Spectroscopy, 62 (2008) 251-257.
  • M.G. Orkoula, C.G. Kontoyannis, C.K. Markopoulou and J.E. Koundourellis, «Validation of a Direct Non-Destructive Quantitative Analysis of Amiodarone Hydrochloride in Angoron® Formulations Using FT-Raman Spectroscopy», Talanta, 73 (2007) 258-261
  • M.G. Orkoula, C.G. Kontoyannis, C.K. Markopoulou and J.E. Koundourellis, «Development of Methodologies Based on HPLC and Raman Spectroscopy for Monitoring the Stability of Lovastatin in Solid State in the Presence of Gallic Acid», J. Pharm. Biomed. Anal., 35 (2004) 1011-1016.