The Secretariat accepts students on weekdays from 12:00 - 13:30

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Study Guide 2019-20

DPHA_3 Pharmaceutical Analysis-Biospectroscopy PDF Print

Instructors: Ch. Kontoyannis, F. Lamari, Μ. Orkoula

Co-ordinator: Μ. Orkoula

Content:

Characteristics of analytical methods-Standard solutions - Validation procedure -Regulatory requirements by Pharmacopoeia and other international organizations. UV-Visible spectroscopy: Introduction, electromagnetic spectrum, absorbance laws and limitations, instrumentation-design and working principle, chromophore concept, auxochromes.

IR, ATR and micro-IR spectroscopy: Basic principles - Molecular vibrations, vibrational frequency, factors influencing vibrational frequencies, sampling techniques, instrumentation, interpretation of spectra, FT-IR, theory and applications.

Raman and micro-Raman spectroscopy: Basic principles - Sampling techniques, instrumentation, interpretation of spectra, theory and applications.

Fluorescence spectroscopy: Basic principles, instrumentation, interpretation of spectra, theory and applications.

Circular dichroism: Basic principles, instrumentation, interpretation of spectra, theory and applications.

Mass spectrometry: Theory, ionization techniques: electron impact ionization, chemical ionization, field ionization, fast atom bombardment, plasma desorption, fragmentation process: types of fission, resolution, interpretation of spectra and applications for identification and structure determination.

Sample pretreatment for analysis with separation techniques.

Electrophoresis: Theory, instrumentation and various techniques (e.g. paper, gel, capillary electrophoresis etc.) applications for analysis pharmaceuticals.

Thin Layer Chromatography: Theory, simple and automated instrumentation. Applications to pharmaceutical analysis.

Liquid and Gas Chromatography (LC and GC): Introduction, instrumentation, different modes of operation. Various detection modes with emphasis on mass spectrometry. Applications to pharmaceutical analysis.