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Study Guide 2019-20

DPHA_A03 Biomolecular NMR and protein architecture PDF Print

Instructors: G. Spyroulias, M. Fousteris

Co-ordinator: G. Spyroulias

Content:

Theory and Principles in NMR Spectroscopy – Strategies in 1Η 1D resonance assignment – Examples & Practical Exercise.
Basics of Structural Bioinformatics, Computational & Structural Biology – Methods, Tools, Data bases & Data mining.
Preparation of biomolecular samples (proteins, RNA, DNA, etc) suitable for NMR studies – Methods in protein/RNA uniform labeling, selective/specific and reverse selective labeling of aminoacids in proteins.
2D homo- & hetero- nuclear NMR Spectroscopy – Methodology and Experimental set-up – Applications in peptides/polypeptides.
3D homo- hetero- nuclear NMR Spectroscopy – Methodology and Experimental set-up – Applications in proteins.
Approaches in NMR studies of biomolecules and biomolecular high molecular weight complexes.
15N-relaxation measurements, H/D exchange and protein dynamics.
Determination of 3D structural models using NMR data.
Study of protein – protein/nucleic acids/RNA/DNA/small molecules interaction through NMR, calculation of Kd, design of bioactive molecules through biomolecular NMR.
NMR in drug design.
NMR spectroscopy in lead discovery and optimization process.
Modern approaches in Structural Biology & Magnetic Resonance Diagnostics -– in cell NMR, NMR-metabolomics, etc.